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If incorrect, please enter your country into the box below, to view site information related to your location. Get access to the best antibodies, discovery platforms, and know-how to advance your diagnostic and therapeutic programs. I agree to the terms and conditions. This kit is not compatible with fixed samples. Stained cells must be measured live. The kits are stable for at least 6 months from receipt. Our Abpromise guarantee covers the use of ab113851 in the following tested applications.
Use at an assay dependent concentration. In combination with other assays, they developed the hypothesis that Dox induces increased ROS expression, leading to increased calcium levels and cell death, and that Met reduces this effect by increasing AMPK expression. Cells were then analyzed on a fluorescent plate reader. TBHP mimics ROS activity to oxidize DCFDA to fluorescent DCF.
Acute Effect of anthracyclines on ROS production in HL60 cells. Labeled HL60 cells were treated with idarubicin or doxorubicin for 4 hours at multiple doses according to the protocol. The dotted line represents the mean of 24 replicates of HL60 cells treated with 0. 4,6,4′-trimethylangelicin shows high anti-proliferative activity on DU145 cells under both UVA and blue light.
Creation of a long-acting nanoformulated dolutegravir. Please let us know so that we can cite the reference in this datasheet. I see media with phenol red has a higher background in this assay. What is the typical background OD seen with phenol red media? In the protocol we recommend to run the assay in the absence of phenol red as it can increase the background. The background seems to be more of a problem on spectrophotometers than on flow cytometers.
What is the vehicle that is used as a negative control treatment for cells instead of treating with tbHP? In this case ter-Buytyl hydroperoxide solution is made up in 5M decane. 5mM decane could be used to treat the control culture, as the TBPH is given at 1000X concentration. Is measurement at 570 nm possible?
The dye has the emission maximum at 529nm. We give 535nm, as the old plate reader with UV lamp can measure this wavelength and this works with the kit. My sample is moving cells – spermatozoa. So it is not easy to take a good picture without fixing the sample. The lab says fixation will cause the dyes to leak from the cells.